Where to Buy Fluorescence Microscopes

Thorlabs manufactures a complete suite of components and systems for multiphoton microscopy, including both turnkey multiphoton systems and the ultrafast lasers that power them. Thorlabs has also introduced the OM6N series of high-speed optical modulators, a new all-reflective technology based on deformable mirrors for modulating excitation laser power to the sample with nearly zero dispersion.

Higher multiphoton microscopy brightness, multiple simultaneous label excitation, and simpler microscopy configuration can be achieved with the FYLA SCH all-fiber laser from FYLA. A new concept in multiphoton microscopy which provides the shortest pulses (15 fs) and the highest peak power at the sample plane. With the enhanced photon flux of the SCH laser, higher two-photon excitation efficiencies at lower average power can be achieved, leading to reduced photodamage to live samples.

A plug and play, compact and robust fiber laser with a broad bandwidth (200 nm) which enables the simultaneous excitation of multiple green and red labels. Tuning is not required which enormously simplifies the optical set-up of the multiphoton microscope and reduces the ownership cost.

Fluorescence microscopy allows highly specific imaging of cellular compartments in a minimally invasive fashion while obtaining the highest contrast. The diffraction-limited and pulsed nature of our SuperK lasers makes it possible to study fast lifetime phenomena. To enable maximum flexibility, our supercontinuum laser sources give you high brightness light anywhere within the VIS and nIR wavelength range (390–2400 nm). Get broadband output or tune to the line you need – all in one source.

Optical microscopes are used to study objects that are too small to be seen with the naked eye. This can be organic cells, semiconductor surfaces, or even single molecules. There are two different approaches in optical microscopy, either detecting near-field (SNOM) or far-field signals emitted by the investigated materials.

There are various methods for optical microscopy that utilize different light sources. For some microscopy techniques a simple white light source is sufficient, whereas more sophisticated methods require lasers as light source. Those can be single-mode diode lasers or multi-laser engines that deliver a continuous laser beam at one or several colors, some techniques rely on short laser pulses with femtosecond duration that can be provided by TOPTICA's ultrafast fiber lasers.

RPMC Lasers offers a selection of IR femtosecond lasers for multi-photon microscopy and a wide range of lasers and wavelengths for confocal fluorescence microscopy, ranging from the UV though the IR. All of these lasers provide a high quality TEM₀₀ beam profile, and a stable power output. Additionally, RPMC Lasers offers a multi-laser combiner which is ideal for coupling multiple excitation lasers to a single microscope. See also our white paper on How Mode-Locked Lasers Affect the Past, Present, and Future of Two-Photon Microscopy!

The MPX-series is a truly turn-key, integrated, compact, easy-to-use, maintenance-free and easy-to-install imaging platform which offers refined multimodality. The user does not need to worry about laser alignment, detection efficiency, non-compatible or misaligned hardware over time. It can easily be operated even by non-experts. The built-in multimodality combination of imaging capabilities allows more features for correlative microscopy of any system available. Once the sample is positioned the user is allowed to switch modalities, resolution and imaging level (micro or macro) without changing the device.